Published Online: September
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Genomic DNA was extracted using a rapid non-enzymatic method (1). PCR amplification of both the loci was achieved by using locus specific primers flanking the repeat region (2,3) and carried out in a Hybaid™ thermal cycler using Taq polymerase (Roche Molecular Diagnostics, Gmbh, Germany). Amplimers were electrophoresed in 6% denaturing urea gel (7M) and analyzed by Fragment manager using ALF DNA Sequencer (Amersham Pharmacia Biotech, Uppasala, Sweden). Allelic ladders for both the loci were developed in our laboratory and used for the correct assignment of the allele sizes.
Stock #: JFS2003115