ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2003
Page Count: 2

Haplotype Frequency Distribution of Two Autosomal Microsatellites (D12S66 and D12S67) Among Five Endogamous Population Groups in India

Abstract

Genomic DNA was extracted using a rapid non-enzymatic method (1). PCR amplification of both the loci was achieved by using locus specific primers flanking the repeat region (2,3) and carried out in a Hybaid™ thermal cycler using Taq polymerase (Roche Molecular Diagnostics, Gmbh, Germany). Amplimers were electrophoresed in 6% denaturing urea gel (7M) and analyzed by Fragment manager using ALF DNA Sequencer (Amersham Pharmacia Biotech, Uppasala, Sweden). Allelic ladders for both the loci were developed in our laboratory and used for the correct assignment of the allele sizes.

Keywords:
forensic science, DNA typing, D12S66, D12S67, haplotype frequency, Indian population

Paper ID: JFS2003115
DOI: 10.1520/JFS2003115
ASTM International is a member of CrossRef.