Volume 48, Issue 6 (November 2003)

    Validation and Uses of a Y-Chromosome STR 10-Plex for Forensic and Paternity Laboratories

    (Received 14 July 2003; accepted 10 July 2003)

    Published Online: November

    CODEN: JFSOAD

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    Abstract

    Y-chromosome short tandem repeats (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of male lineages. The present study validated a Y-STR 10-plex on the ABI PRISM® 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid Cellmark. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. This study demonstrated that the 10-plex is sensitive to 0.125 ng of input DNA and that female samples mixed with male samples did not interfere with Y-STR haplotyping. In a sample of 525 males, there were three instances of locus multiplication, two at DYS19 and one at DYS435. Overall, haplotype diversity was 0.996, suggesting that the 10-plex can effectively distinguish among male lineages.


    Stock #: JFS2003114

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003114

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    Author
    Title Validation and Uses of a Y-Chromosome STR 10-Plex for Forensic and Paternity Laboratories
    Symposium , 0000-00-00
    Committee E30