ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 November 2003
Page Count: 9

Validation and Uses of a Y-Chromosome STR 10-Plex for Forensic and Paternity Laboratories
(Received 14 July 2003; accepted 10 July 2003)

Abstract

Y-chromosome short tandem repeats (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of male lineages. The present study validated a Y-STR 10-plex on the ABI PRISM® 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid Cellmark. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. This study demonstrated that the 10-plex is sensitive to 0.125 ng of input DNA and that female samples mixed with male samples did not interfere with Y-STR haplotyping. In a sample of 525 males, there were three instances of locus multiplication, two at DYS19 and one at DYS435. Overall, haplotype diversity was 0.996, suggesting that the 10-plex can effectively distinguish among male lineages.

Keywords:
forensic science, DNA typing, Y-chromosome, short tandem repeat, DYS436, DYS439, DYS435, DYS19, DYS460, Y-GATAH4, DYS391, DYS392, DYS438, DYS437

Paper ID: JFS2003114
DOI: 10.1520/JFS2003114
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