Volume 48, Issue 5 (September 2003)
Distribution of Allele Frequencies of One VNTR and Two STR Loci in Five Population Groups of South India
DNA was extracted by using a non-enzymatic method (1) from blood of normal, healthy, and random individuals. Amplification of each of the three loci was performed using locus specific primers and PCR conditions were as described by Lichter et al. (2) for DRD4 and Perez-lezaun et al. (3) for STR markers. Forward primer of both the STR markets was labeled with fluorescent Cy5™dye amidite (Amersham Pharmacia Biotech Pvt.Ltd, Sweden). Amplification was carried out in 25 µL PCR reaction mixture containing 25 ng of genomic DNA in Eppendorf Gradient Master Cycler for all three of the loci.