ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2003
Page Count: 2

Microsatellite Markers in the Population from Rio Grande Do Norte, Northeastern Brazil

Abstract

Samples of venous blood were collected using the anticoagulant ACD (citric acid 0.038 M; tribasic sodium citrate 0.075 M; dextrose 0.033 M), from 247 non-related, healthy individuals from the population of the State of Rio Grande do Norte, in Northeastern Brazil. The DNA was extracted by rapid mini salting-out and digestion with proteinase K (1). The PCR was performed in a Perkin-Elmer 2400 thermal cycler, using amplification protocols described in Promega Manual (Madison, WI). The amplified products underwent electrophoresis in a denaturing polyacrylamide gel in a manual sequencer from Amershan Pharmacia (SQ3 Sequencer). The statistical analysis was performed using the Genetic Data Analysis (2) and the PowerStat (3) softwares. The complete dataset is available in www.nlink.com.br/mauricio.

Keywords:
forensic science, population genetics, DNA typing, HUMTH01, TPOX, CSF1PO, LPL, F13B, D16S539, D7S820, D13S317

Paper ID: JFS2003102
DOI: 10.1520/JFS2003102
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