Published Online: September
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Samples of venous blood were collected using the anticoagulant ACD (citric acid 0.038 M; tribasic sodium citrate 0.075 M; dextrose 0.033 M), from 247 non-related, healthy individuals from the population of the State of Rio Grande do Norte, in Northeastern Brazil. The DNA was extracted by rapid mini salting-out and digestion with proteinase K (1). The PCR was performed in a Perkin-Elmer 2400 thermal cycler, using amplification protocols described in Promega Manual (Madison, WI). The amplified products underwent electrophoresis in a denaturing polyacrylamide gel in a manual sequencer from Amershan Pharmacia (SQ3 Sequencer). The statistical analysis was performed using the Genetic Data Analysis (2) and the PowerStat (3) softwares. The complete dataset is available in www.nlink.com.br/mauricio.
Stock #: JFS2003102