Volume 48, Issue 4 (July 2003)
Allele Frequencies for Two STR Loci D9S1118 and D20S161 in Chinese Han Ethnic Group in Chengdu
Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/D9S1118.htm. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). The allele classification for each STR locus was based on the number of repeat motifs according to the recommendations of the International Society of Forensic Haemogenetics (3), which now is known as the Interna tional Society of Forensic Genetics, ISFG. The alleles at D20S161 locus was named according to Susukida et al. (4). Data were analyzed using POWERSTATS program (5). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (6) and no deviation from Hardy-Weinberg equilibrium was observed.