Abstract

Blood samples were collected from 109 unrelated males of Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). The volume of PCR reaction for each locus was 37.5 µL, which contained 2-10 ng human genome, 1 X Taq buffer, 1.5 mM MgCl2, 1.6 . g/mL BSA, 200 RM each dNTP (Pharmacia Biotech, Sweden), 1.5 U Taq polymerase (Promega Corporation, Madison, WI), 0.3 RM each primer. PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer, Foster City, CA) with pre-denaturing for 3 min at 94°C, 36 cycles of denaturing for 30 s at 94°C, annealing for 60 s at 61°C and extension for 30 s at 72°C. The amplicons were analyzed by horizontal nondenaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Alleles were designated according to recommendation of the DNA commission of the International Society of Forensic Genetics (3). Data of population genetics and forensic science were analyzed according to Hou's method (4).

Author Information

Wang, XD
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Dai, HL
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Hou, YP
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Tang, JP
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Zhu, QF
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Ji, Q
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Dong, JG
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Li, YB
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Wu, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Zhang, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences), Sichuan, P. R. China
Pages: 2
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Stock #: JFS2003068
ISSN: 0022-1198
DOI: 10.1520/JFS2003068