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Volume 49, Issue 1 (January 2004)

ISSN: 0022-1198
CODEN: JFSCA
Published Online: 17 December 2003
Page Count: 3


Assignment of Paternity in a Judicial Dispute Between Two Neighbor Holstein Dairy Farmers
Giovambattista, G
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, UNLP,

Lirón, JP
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, UNLP,

Ripoli, MV
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, UNLP,

García, PP
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, UNLP,

(Received 24 February 2003; accepted 4 September 2003)

Abstract

DNA profiling was used as evidence to assign paternity in a dispute between two neighbors in a judicial case of undue appropriation of cattle offspring from five alleged Holstein sires. Five offspring were genotyped using ten genetic markers (nine microsatellites and the BOLA-DRB3 locus). The computer program CERVUS was used to estimate the LOD score values and the confidence of paternity assignments. The results presented here show that three out of five paternity cases were assigned at 95% of confidence to a single sire with a LOD score ranging from 2.53 to 3.55. A fourth male was assigned using its Δ value. Finally, all alleged sires were excluded from the paternity of the fifth offspring, probably due to the existence of an non-sampled male in the studied population.We concluded that the likelihood-based approach, included into CERVUS program, was a powerful tool in cattle kinship analysis when dealing with judicial dispute particularly when the dam's genotype was absent, allowing the assignments of paternity at 95% level of confidence in situations usually used by dairy and beef cattle producers in Argentine (e.g., multi-sire pasture mating).



Keywords:
forensic science, paternity, DNA typing, microsatellites, BOLA-DRB3, Holstein

Paper ID: JFS2003061
DOI: 10.1520/JFS2003061
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Author Title Assignment of Paternity in a Judicial Dispute Between Two Neighbor Holstein Dairy Farmers Symposium , 0000-00-00 Committee E30