Validation and Implementation of the PowerPlex® 16 BIO System STR Multiplex for Forensic Casework

    Volume 49, Issue 1 (January 2004)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 17 December 2003

    Page Count: 10


    Glessner, AL
    Pennsylvania State Police DNA Laboratory, Greensburg, PA

    Amin, AS
    The Bode Technology Group, Springfield, MD

    Heidebrecht, BJ
    Maryland State Police Crime Laboratory, MD

    Braunstein, CA
    Maryland State Police Crime Laboratory, MD

    Crouse, CA
    Palm Beach County Sheriff's Office, West Palm Beach, FL

    Tomsey, CS
    Pennsylvania State Police DNA Laboratory, Greensburg, PA

    Soberalski, C
    Indiana State Police, IN

    Freeman, DA
    North Carolina State Bureau of Investigation, NC

    Douglas, EK
    The Bode Technology Group, Springfield, MD

    Kist, FG
    Pennsylvania State Police DNA Laboratory, Greensburg, PA

    Ban, JD
    Virginia Division of Forensic Science, Richmond, VA

    Schumm, JW
    The Bode Technology Group, Springfield, MD

    Mihalacki, LR
    Pennsylvania State Police DNA Laboratory, Bethlehem, PA

    Pablo, L
    Virginia Division of Forensic Science, Richmond, VA

    Bruesehoff, N
    Indiana State Police, IN

    Greenspoon, SA
    Virginia Division of Forensic Science, Richmond, VA

    Long, TM
    Maryland State Police Crime Laboratory, MD

    (Received 8 February 2003; accepted 13 September 2003)

    Abstract

    The PowerPlex® 16 BIO multiplex short tandem repeat (STR) system contains the 13 CODIS loci (FGA, TPOX, D8S1179, vWA, D18S51, D21S11, TH01, D3S1358, CSF1PO, D16S539, D7S820, D13S317, and D5S818), plus two pentanucleotide repeat loci (Penta D and Penta E) and the sex-identifying locus, Amelogenin. The PowerPlex® 16 BIO System is optimized for use with the Hitachi FMBIO® gel imaging systems. A consortium of seven independent laboratories collaborated to perform the studies defined by the FBI standards for performing a developmental validation, including the evaluation of sample concordance, percent stutter determination, nonprobative casework, precision, sensitivity, mixture determination, effect of substrates, the impact of environmental insults, and species specificity. All samples tested for concordance were consistent except for one sample from the Virginia Division of Forensic Science database that displayed discordance at D13S317, a locus whose primer sequence was altered. Stutter values were comparable to those of other STR multiplex systems, the precision was comparable to other multiplexes analyzed by gel electrophoresis, the DNA profiles were unchanged by the substrate upon which the blood samples were placed, and the nonprobative casework samples re-typed for the PowerPlex® 16 BIO System were consistent with previous typing results. When greater than 0.125 ng of DNA was placed into the PowerPlex® 16 BIO System amplification reaction, a full profile was generated by all laboratories. The mixture study results were comparable to those reported for other multiplex systems, the environmental study demonstrated a loss of larger molecular weight loci when samples were incubated at elevated temperatures for a prolonged period of time, and the only notable cross species hybridization was observed with primate DNA samples. This extensive validation work performed demonstrates that the PowerPlex® 16 BIO System provides STR data of a quality comparable with other PowerPlex® STR multiplex kits as well as other widely used STR multiplexes and is thus suitable for evidentiary casework analysis as well as database sample profiling.


    Paper ID: JFS2003031

    DOI: 10.1520/JFS2003031

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    Author
    Title Validation and Implementation of the PowerPlex® 16 BIO System STR Multiplex for Forensic Casework
    Symposium , 0000-00-00
    Committee E30