Volume 49, Issue 3 (May 2004)

    Screening Postmortem Whole Blood for Oxycodone by ELISA Response Ratios

    (Received 31 January 2003; accepted 31 December 2003)

    Published Online: March

    CODEN: JFSOAD

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    Abstract

    The objective of this study was to investigate the accuracy of screening postmortem whole blood for oxycodone using the ratio of the oxycodone immunoassay response to the response for the specimen obtained with a general opiate-class immunoassay. Fifty eight specimens which were negative for opiates and 158 postmortem whole blood specimens positive for opiates including 66 specimens known to contain oxycodone were assayed. Specimens were diluted 1:5 with assay buffer and analyzed by both the Neogen Oxymorphone/Oxycodone ELISA and the Neogen Opiate Group ELISA (Neogen Corporation, Lexington KY). The oxycodone equivalents in ng/mL from the Oxymorphone/Oxycodone ELISA were divided by the morphine equivalents in ng/mL from the Opiates ELISA to obtain an Oxycodone/Opiates Response Ratio. This ratio was compared with the GC/MS data for all specimens and for opiate positive specimens. Receiver Operating Characteristic (ROC) analysis suggested that optimum relative response ratio was 2.0. The sensitivity of the ELISA response ratio for the presence of oxycodone at a response ratio cutoff of 2.0 was 89.4% ± 3.8% and the specificity was 88.1% ± 3.2%. Specimens with a ratio of 2.0 or higher had a greater than 50% probability (positive predictive value) of containing oxycodone in a population with a greater than 15% prevalence of oxycodone.


    Author Information:

    McCutcheon, JR
    Office of the Medical Examiner of Travis County, Austin, TX

    DeCicco, L
    Analytical Research Laboratories, Oklahoma City, OK.

    Kemp, P
    Office of the Chief Medical Examiner, Oklahoma City, OK

    Kupiec, T
    Analytical Research Laboratories, Oklahoma City, OK.

    Spiehler, VR
    Spiehler and Associates, Newport Beach, CA


    Stock #: JFS2003028

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003028

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    Author
    Title Screening Postmortem Whole Blood for Oxycodone by ELISA Response Ratios
    Symposium , 0000-00-00
    Committee E30