Allelic Frequency of Six STR Loci in Five Colombian Cities

    Volume 48, Issue 4 (July 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 2003

    Page Count: 2


    Abstract

    Blood samples were obtained at blood banks in large cities such as Bogota, Cali, Cucuta, Barranquilla, and Medellin by venopuncture with EDTA anticoagulant. They were informed of the research and so they donated their blood voluntarily before signing a written consent document. Genomic DNA was extracted by using phenolchloroform methods with some modifications for anticoagulated blood samples (1). The ion-exchange resin method (CHELEX 100) was also used according to the established protocol (2). DNA samples were amplified through PCR reactions using the multiplex kit (CTT and FFv from Promega Corp., GenePrint™, STR Systems, Silver stain detection Madison, WI). The PCR products from the six STR loci were analyzed using vertical electrophoresis protocol; minor modifications were introduced to fit available equipment. An electrophoresis camera (C.B.S. Scientific Co., Del Mar, adjustable CA, ASG 250-400 U.S. version) was used for 6% polyacrylamide denaturing gels in all six STR markers. Then, silver staining was applied according to the method described by Bassam BJ (3). Amplification was made in a LABLINE, 212S-model


    Paper ID: JFS2002444

    DOI: 10.1520/JFS2002444

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    Author
    Title Allelic Frequency of Six STR Loci in Five Colombian Cities
    Symposium , 0000-00-00
    Committee E30