Volume 48, Issue 3 (May 2003)
Genetic Variation for 7 STR Loci in a Colombian Population (Department of Valle del Cauca)
Whole blood samples were obtained from 81—461 unrelated Colombian individuals from the parentage testing routinely done at the Laboratory of Immunology and Immunogenetics, Hospital Universitario del Valle in Cali, Colombia, previous informed consent. Genomic DNA was extracted from 1 mL of EDTA anticoagulated peripheral blood using the salting-out methodology. After DNA quantitation by spectrophotometry, 1—2 ng of DNA were amplified using the commercial kits AmpFℓSTR Cofiler (PE Biosystems, Foster City, CA), Geneprint Fluorescent STR Multiplex F13A01, FES/FPS, F13B, LPL (FFFL) and PowerPlex 16 (Promega Corporation, Madison, WI), following the manufacturer's instructions. The amplified products were separated and detected using the ABI 310 DNA sequencer (PE Biosystems, Foster City, CA). Alleles were classified according to the recommendations of the ISFH (1). Statistical analysis was performed using the GDA program (2). Statistical parameters such as power of discrimination (PD) and a priori chance of exclusion (CE) for each loci were estimated as described by Huston (3). Also we calculated the polymorphic information content (PIC) according to Botstein et al. (4). The Hardy-Weinberg equilibrium for each loci and linkage disequilibrium were verified using the GDA program. We did not find significant deviation from Hardy-Weinberg equilibrium at all loci. The complete data set is available to any interested researcher upon request from the corresponding author.