Distributions of Allelic Frequencies and Haplotypes of Two Novel Y-Chromosome STR in a Chinese Population

    Volume 48, Issue 2 (March 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 2003

    Page Count: 1


    Abstract

    Whole blood obtained by venipuncture from 107 unrelated males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 μL, containing 2–4 ng human genome DNA, 200 μM each dNTP (Pharmacia, Sweden), 1.5μ Taq polymerase (Promega, Madison, WI), 3.75 μL 10 × buffer, Mg2+ 1.5 mM, 1.6 μg/mL BSA, 0.3 μM each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3).


    Paper ID: JFS2002381

    DOI: 10.1520/JFS2002381

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    Title Distributions of Allelic Frequencies and Haplotypes of Two Novel Y-Chromosome STR in a Chinese Population
    Symposium , 0000-00-00
    Committee E30