Volume 48, Issue 5 (September 2003)
A Novel Multiplex PCR System Consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445
We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs-DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 µL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.