ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2003
Page Count: 3

A Novel Multiplex PCR System Consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445
(Received 29 March 2003; accepted 29 March 2003)

Abstract

We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs-DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 µL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.

Keywords:
forensic science, DNA typing, DYS441, DYS442, DYS443, DYS444, DYS445, multiplex polymerase chain reaction, short tandem repeat, semen stain

Paper ID: JFS2002379
DOI: 10.1520/JFS2002379
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