Identification of DYS385 Allele Variants by Using Shorter Amplicons and Northern Thai Haplotype Data

    Volume 48, Issue 5 (September 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 2003

    Page Count: 5


    (Received 16 May 2003; accepted 10 May 2003)

    Abstract

    Primers currently used for amplification of locus DYS385 on the Y chromosome give quite long amplicons that are difficult to resolve on native polyacrylamide gels. We therefore designed new primers that give much shorter products for easy separation. With this system, two allele variants that were characterized by sequencing as 14.1 (GAAA -* GAAAA) and 14.2 (GA insertion), were identified in two unrelated Northern Thai men due to their decreased mobility. Interestingly, initial capillary electrophoresis mistyped the 14.1 variant amplified with the traditional primers as allele 14. Forty different haplotypes were found in 147 Northern Thais with a haplotype diversity of 0.9430. Haplotype 13-18 (frequency 0.136) is the most frequent one; for comparison; haplotype 14-18 (frequency 0.074) was found to be the most common in a population from Bangkok (n = 95) in an earlier study. This striking difference within Thailand's population probably results from genetic differences in the founding populations.


    Paper ID: JFS2002371

    DOI: 10.1520/JFS2002371

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    Title Identification of DYS385 Allele Variants by Using Shorter Amplicons and Northern Thai Haplotype Data
    Symposium , 0000-00-00
    Committee E30