Volume 48, Issue 2 (March 2003)

    Haplotype Frequencies of Three Y-Chromosome STR Loci in Tibetan Ethnic Group of Chinese Population

    Published Online: March

    CODEN: JFSOAD

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    Abstract

    A total of 101 blood samples were collected from unrelated males of Tibetan ethnic group in Lasa of China. DNA was extracted using Chelex method (1). The allelic variation at the three Y-STR loci named as DYS434, DYS443, and DYS456, were analyzed by PCR amplication with multiplex system. Multiplex PCR amplifications were performed in a 37.5 μL containing 2–4 ng human genome DNA, 7.5 μL dNTP (1 mmol/mL), 3μ, Taq polymerase, 3.75 μL 10 × buffer (Mg2+ 1.5 mmol/l), 3.75 μL BSA (1.6 μg/mL), 0.3 μL each primers (50 nmol/mL).


    Stock #: JFS2002370

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002370

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    Author
    Title Haplotype Frequencies of Three Y-Chromosome STR Loci in Tibetan Ethnic Group of Chinese Population
    Symposium , 0000-00-00
    Committee E30