Published Online: March
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A total of 101 blood samples were collected from unrelated males of Tibetan ethnic group in Lasa of China. DNA was extracted using Chelex method (1). The allelic variation at the three Y-STR loci named as DYS434, DYS443, and DYS456, were analyzed by PCR amplication with multiplex system. Multiplex PCR amplifications were performed in a 37.5 μL containing 2–4 ng human genome DNA, 7.5 μL dNTP (1 mmol/mL), 3μ, Taq polymerase, 3.75 μL 10 × buffer (Mg2+ 1.5 mmol/l), 3.75 μL BSA (1.6 μg/mL), 0.3 μL each primers (50 nmol/mL).
Stock #: JFS2002370