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    Volume 48, Issue 2 (March 2003)

    HLA-DQA1 and Polymarker Allele Frequencies in Peru

    Published Online: March

    CODEN: JFSOAD

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    Abstract

    Whole blood samples were obtained after informed consent from 100 unrelated Peruvian donors. Genomic DNA was extracted by a standard phenol/chloroform extraction procedure. The extracted DNA was quantitated by slot-blot hybridization using the QuantiblotR Human DNA Quantitation kit (PE-Biosystems, Foster City, CA). The samples were typed for HLA-DQA1/PM loci by using the Amplitype PM and DQA1 kit following manufacturer's instructions (PE-Biosystems, Foster City, CA). Exact tests were performed by using the computer program GDA for checking the Hardy-Weinberg expectations (1). Data were analyzed by Power-Stats (2). The complete data set is available to any interested researcher upon request from Oscar Garcia, Ph.D., Area de Laboratorio Ertzaintza, Av. Montevideo 3, 48002-Bilbao, Spain.


    Stock #: JFS2002352

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002352

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    Author
    Title HLA-DQA1 and Polymarker Allele Frequencies in Peru
    Symposium , 0000-00-00
    Committee E30