Volume 48, Issue 2 (March 2003)
Frequencies at CD4, FES, and F13A1 Microsatellite Loci in Central-Southern Sardinia (Italy)
DNA samples were extracted and purified by salting out procedure, precipitated by absolute ethanol and quantified by 1% agarose gel. The DNAs were amplified by Perkin Elmer PCR system 2400 using standard PCR protocols (2-4). Amplified products were separated in denaturing acrylamide gel using an Automated Laser Fluorescent apparatus as previously described (5). HardyWeinberg tests and analysis of genotypic disequilibrium between unlinked loci were carried out by using the Genepop software (6). The results obtained are shown in Table 1. It is to be noted that in all single-locus and multilocus tests, the p-value was statistically insignificant. Furthermore, we calculated the linkage disequilibrium between genotypes of independent loci. Among the possible three pairs of loci, only one case of statistically significant p-value was obtained (FES/F13A1; p = 0.024). Complete data are available at the e-mail address of the corresponding author upon request.