ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 2003
Page Count: 6

Analysis of Human Fecal Material for Autosomal and Y Chromosome STRs
(Received 6 April 2003; accepted 5 April 2003)

Abstract

Human stool samples from eight volunteers were stored under various conditions and extracted by three different procedures. Fecal material and tissue paper soiled with fecal material obtained from a crime scene were also extracted. Extracted DNA was amplified using the AmpF€STR® Profiler Plus™, AmpFℓSTR® COfiler™, and the AmpF€STR® Identifiler™ PCR amplification kits for the detection of the autosomal STR allelic patterns. DNA extracted from the male volunteers and from the soiled tissue paper evidence sample was also amplified using the Y-PLEX™6 and Y-PLEX™5 amplification kits. Analysis of the amplified products was carried out by capillary electrophoresis on the ABI PRISM® 310 Genetic Analyzer. Autosomal and Y-STR profiles obtained from the fecal material were concordant with the results from the donors' buccal swabs.

Keywords:
forensic science, DNA typing, fecal material, polymerase chain reaction, short tandem repeats, DYS393, DYS19, DYS389II, DYS390, DYS391, DYS385, DYS389I, DYS439, DYS438, DYS392, D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, amelogenin

Paper ID: JFS2002348
DOI: 10.1520/JFS2002348
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