Population Data for Seven Y Chromosome STR Loci from Three Different Population Groups Residing in Connecticut

Volume 48, Issue 2 (March 2003)

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ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 March 2003
Page Count: 3

Population Data for Seven Y-Chromosome STR Loci from Three Different Population Groups Residing in Connecticut

Abstract

The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recommended procedures. The quantity of human DNA was determined by slot blot hybridization using the QuantiBlot kit (Applied Biosystems, Foster City, CA) and following the manufacturer's recommended protocols. The Y-PLEX™6 system (ReliaGene Technologies, Inc., New Orleans, LA) was used for the amplification of the Y short tandem repeat (STR) loci. PCR amplification and the analysis of amplified product were performed as recommended by the manufacturer and as described in Sinha et al. (1).

Keywords:
forensic science, Y-chromosome, short tandem repeats, DNA typing, human identification, Y-STR, Y-PLEX, DYS393, DYS19, DYS389II, DYS390, DYS391,, DYS385

Paper ID: JFS2002345
DOI: 10.1520/JFS2002345
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Author Title Population Data for Seven Y-Chromosome STR Loci from Three Different Population Groups Residing in Connecticut Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 48, Issue 2 (March 2003) Click here to download this paper now for $25 PDF (17K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Published Online: 1 March 2003 Page Count: 3 Population Data for Seven Y-Chromosome STR Loci from Three Different Population Groups Residing in Connecticut Abstract The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recommended procedures. The quantity of human DNA was determined by slot blot hybridization using the QuantiBlot kit (Applied Biosystems, Foster City, CA) and following the manufacturer's recommended protocols. The Y-PLEX™6 system (ReliaGene Technologies, Inc., New Orleans, LA) was used for the amplification of the Y short tandem repeat (STR) loci. PCR amplification and the analysis of amplified product were performed as recommended by the manufacturer and as described in Sinha et al. (1). Keywords: forensic science, Y-chromosome, short tandem repeats, DNA typing, human identification, Y-STR, Y-PLEX, DYS393, DYS19, DYS389II, DYS390, DYS391,, DYS385 Paper ID: JFS2002345 DOI: 10.1520/JFS2002345 ASTM International is a member of CrossRef. Author Title Population Data for Seven Y-Chromosome STR Loci from Three Different Population Groups Residing in Connecticut Symposium , 0000-00-00 Committee E30