Volume 48, Issue 1 (January 2003)
French Allele Frequencies and Haplotypes of Nine Y-specific STRs
Blood samples or buccal swabs were collected from 100 Caucasian males living in Strasbourg, after obtaining their informed consent. After DNA extraction using the Cleanmix kit (Talent), all males were analyzed at nine STR loci. The six Y-STR loci DYS19, DYS389-II, DYS390, DYS391, DYS393 and, DYS385 were coamplified in a multiplex reaction using the Y-Plex6™ kit according to the manufacturers' recommendations (ReliaGene Technologies Inc.). The three others (DYS389-I, DYS392, and YCAII) were amplified by singleplex PCR. Primer sequences were those described by de Knijff et al. (1), except for the loci DYS389-I which was amplified using the primer sequence described by Schultes et al. (2). Amplification products were analyzed by capillary electrophoresis on an ABI Prism 3100 Genetic analyzer (Applied Biosystems). Fragment sizes were determined automatically using the GeneMapper software and typed by comparison with an allelic ladder (provided in the kit or obtained by the mixture of previously sequenced samples for the most common alleles). The allele nomenclature was the one recommended by the International Society of Forensic Genetics (3). Data have been contributed to the YHRD and can be searched at www.ystr.org/europe.