You are being redirected because this document is part of your ASTM Compass® subscription.
    This document is part of your ASTM Compass® subscription.

    Volume 48, Issue 1 (January 2003)

    Allele Frequencies for Six STR Loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a Population Sample of North Italy

    Published Online: January

    CODEN: JFSOAD

      Format Pages Price  
    PDF 2 $25   ADD TO CART

    Cite this document

    X Add email address send
    X
      .RIS For RefWorks, EndNote, ProCite, Reference Manager, Zoteo, and many others.   .DOCX For Microsoft Word



    Abstract

    Whole blood samples were collected from 170 unrelated healthy individuals living in the North Italy. Genomic DNA was extracted using GenomicPrep Blood DNA Isolation Kit (Amersham-Pharmacia-Biotech, Milano, Italy) and its concentration was determined spectrophotometrically. Two to five ng of target DNA was amplified in singleplex for FGA and D8S1179, while duplex amplification was adopting for D13S317/D16S539 and D5S818/D7S820. PCR amplification conditions were the same for all six loci and the program of amplification were carried out in a GeneAmp® PCR System 9700 Thermal Cycler (PE-Biosystems, Foster City, CA). The amplified products were separated and detected using the A.L.F. express DNA sequencer (Pharmacia-Biotech, Uppsula, Sweden). Allele designation was established following the recommendations of the DNA commission of the ISFH (1). Statistical analysis was performed as previously reported (2) by a computer program made from the authors using Excel spread sheets.


    Author Information:

    De Leo, D
    Postdoctoral Fellow, University of Verona, Verona,

    Turrina, S
    Postdoctoral Fellow, University of Verona, Verona,


    Stock #: JFS2002305

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002305

    ASTM International
    is a member of CrossRef.

    Author
    Title Allele Frequencies for Six STR Loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a Population Sample of North Italy
    Symposium , 0000-00-00
    Committee E30