ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 2

Allele Frequencies for Six STR Loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a Population Sample of North Italy
De Leo, D
Postdoctoral Fellow, University of Verona,

Turrina, S
Postdoctoral Fellow, University of Verona,

Abstract

Whole blood samples were collected from 170 unrelated healthy individuals living in the North Italy. Genomic DNA was extracted using GenomicPrep Blood DNA Isolation Kit (Amersham-Pharmacia-Biotech, Milano, Italy) and its concentration was determined spectrophotometrically. Two to five ng of target DNA was amplified in singleplex for FGA and D8S1179, while duplex amplification was adopting for D13S317/D16S539 and D5S818/D7S820. PCR amplification conditions were the same for all six loci and the program of amplification were carried out in a GeneAmp® PCR System 9700 Thermal Cycler (PE-Biosystems, Foster City, CA). The amplified products were separated and detected using the A.L.F. express DNA sequencer (Pharmacia-Biotech, Uppsula, Sweden). Allele designation was established following the recommendations of the DNA commission of the ISFH (1). Statistical analysis was performed as previously reported (2) by a computer program made from the authors using Excel spread sheets.

Keywords:
forensic science, DNA typing, population genetics, D5S818, D7S820, D8S1179, D13S317, D16S539, FGA, North Italy

Paper ID: JFS2002305
DOI: 10.1520/JFS2002305
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