Published Online: July
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Blood samples were collected from 176 unrelated Egyptian individuals in EDTA-coated vacutainer tubes. Of these, 148 individuals were Muslims and 28 Christian. Informed consent was obtained at the time of blood collection. DNA was extracted by organic extraction (Phenol/chloroform/Isoamyl alcohol) followed by ethanol precipitation (1). The quantity of DNA was determined using ACES 2.0 quantiblot kit (GIBCO-BRL Gaithersburg, MD). Promega PowerPlex 1.2 and PE Profiler Plus kits were used to amplify DNA from the Muslim and Christian populations, respectively. Amplified products were electrophoresed on an ABI 310 Genetic Analyzer (ABI Systems, Foster City, CA). Data were analyzed using DNA typing program written by Chakraborty and Zhong (2).
Stock #: JFS2002297