Volume 48, Issue 1 (January 2003)
Allele Frequencies of Nine STR Loci in Chinese She Ethnic Group from Zhejiang, China
D21S11, D18S51, D5S818, D13S317, D7S820 Blood samples were collected from 120 unrelated individuals of Chinese She ethnic group in Zhejiang province, China. DNA was extracted from blood samples using Chelex method (1). The PCR amplification of the nine STR loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820) was carried out by using reagents provided in the AmpFfSTR Profiler Plus™ kit (PE-Biosystems, Foster City, CA) and using a GeneAmp PCR System 9600 (PE-Biosystems, Foster City, CA) according to the technical manual. The amplified products were analyzed by ABI Prism™ 310 Genetic Analyzer (PE-Biosystems, Foster City, CA) according to the manufacturer's recommendation protocol. Data of population genetics and forensic science were analyzed using POWERSTATS program (2). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (3) and no deviation from Hardy-Weinberg equilibrium was observed.