Fingerprints as Evidence for a Genetic Profile: Morphological Study on Fingerprints and Analysis of Exogenous and Individual Factors Affecting DNA Typing

    Volume 48, Issue 3 (May 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 May 2003

    Page Count: 7


    (Received 31 December 2003; accepted 31 December 2003)

    Abstract

    Material recovered from 374 fingerprints left by eleven laboratory workers on three different substrates (glass, wood, metal) at a standard pressure time of 30 s, with and without preliminary handwashing, was submitted to morphological, quantitative, and type analysis. Morphological and agarose-gel electrophoresis analysis showed that a non-negligible amount of epidermal corneal cells presented apoptotic alterations. The quantity of DNA recovered from fingerprints ranged between 0.04 to 0.2 ng, and in a significant number of experiments no DNA was detected. Handwashing reduced the amount of DNA recovered from fingerprints. The "shedder status" of the donor was a very important factor, causing inter-individual variations in the amount of DNA left by fingerprints. Spurious alleles from laboratory-based and secondary transfer contamination, stutters, and other artifacts described when analyzing low-copy-number DNA and capable of affecting correct profiles were observed.


    Paper ID: JFS2002260

    DOI: 10.1520/JFS2002260

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    Title Fingerprints as Evidence for a Genetic Profile: Morphological Study on Fingerprints and Analysis of Exogenous and Individual Factors Affecting DNA Typing
    Symposium , 0000-00-00
    Committee E30