Volume 48, Issue 1 (January 2003)
Allele Frequencies for Two STR Loci D11S1977 and D22S444 in Chinese Population
Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). Reaction condition of PCR amplification can be accessed at http://www.legalmed.org/ dna/D11S1977.htm. The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system, and visualized by silver staining (2,3). Data of population genetics and forensic science were analyzed using POWERSTATS program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (5) and no deviation from Hardy-Weinberg equilibrium was observed. The complete data can be accessed at http://www.legalmed.org/dna/D11S1977.htm.