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The samples were collected from 168 unrelated Turkish individuals randomly selected from criminal cases. The DNA was extracted from fresh blood leucocytes and blood stains following the published methods (1,2). For each PCR mix 2-25 ng of DNA was added to the PCR mix. All the CTTV loci were amplified in one reaction tube. Amplification of multiplex loci was done with the help of GenePrint STR system CTTV kit in accordance of the manufacturer's (Promega Corp., Madison, WI) recommendations. Separations of the amplified alleles were performed by ABI Prism 310 Genetic Analyzer. The exact test (3) for the presence of HardyWeinberg equilibrium was carried out by the TFPGA (4) program. Heterozygosity (5) values (expected and observed) for the populations were calculated by employing GENETIX (6) program.
Middle East Technical University (METU), Ankara, Turkey
Department of Biology, Ankara,
Stock #: JFS2002248