ISSN: 0022-1198
CODEN: JFSCA
Published Online: 23 December 2003
Page Count: 5

Mutation in the STR Locus D21S11 of Father Causing Allele Mismatch in the Child
Reddy, AD
Centre for Cellular and Molecular Biology,

Thangaraj, K
Centre for Cellular and Molecular Biology,

Singh, L
Centre for Cellular and Molecular Biology,

(Received 8 July 2002; accepted 20 September 2003)

Abstract

We analyzed a case of paternity dispute with 15 autosomal STR loci and found a mismatch in one of the alleles of the locus D21S11 in the child. The composition of the alleles of this locus in the mother, suspicious father, and child were 29/32, 29/29, and 29/30, respectively. The combined paternity index (2.4×10¹⁰) and paternity probability (0.9999) suggest that the suspicious father is the biological father of the child. Further analysis of 6 Y chromosome STR loci revealed matching of all the Y chromosomal alleles of the child with that of the suspicious father. Since there was a perfect match of all the paternal alleles inherited (15 autosomal and 6 Y chromosomal) in the child with that of the suspicious father except the allele D21S11, it is suggested that this might be a case of mutation. Cloning and sequencing of all the alleles of the locus D21S11 of the suspicious father, mother, and the child helped in determining that the suspicious father contributed the mutated allele.

Keywords:
forensic science, DNA typing, short tandem repeats, mutation, Y chromosome, paternity test, paternity index, plasmid cloning, DNA sequencing, Penta E, D18S51, D21S11, THO1, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1PO, D16S539, D7S820, D13S317, D5S818, DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS393

Paper ID: JFS2002236
DOI: 10.1520/JFS2002236
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