Allele Frequency of Eight Y-Chromosome STR Loci in Oriya Population of India

    Volume 48, Issue 1 (January 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2003

    Page Count: 8


    Chainy, GBN
    Utkal University, Bhubaneswar,

    Sahoo, S
    Central Forensic Science Laboratory, Kolkata,

    Kashyap, VK
    Central Forensic Science Laboratory, Kolkata,

    Abstract

    DNA was isolated from blood samples obtained from 150 healthy male individuals by standard phenol/chloroform procedure (3). Custom-made primers were labeled with 5'-FAM and JOE fluorescent dyes. PCR amplification for all the eight Y-STRs namely, DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393, was carried out with a 25-ng template DNA in two multiplex reactions, standardized in the laboratory, using primers as described elsewhere (4,5). Amplified PCR products were run on a 5% polyacrylamide gel using a ABI 377 Automated DNA Sequencer. Sizing of the products was done using the ABI 377 Genescan Analysis Software version (3.1).


    Paper ID: JFS20022310

    DOI: 10.1520/JFS20022310

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    Title Allele Frequency of Eight Y-Chromosome STR Loci in Oriya Population of India
    Symposium , 0000-00-00
    Committee E30