Volume 48, Issue 1 (January 2003)
Allele Frequency of Eight Y-Chromosome STR Loci in Oriya Population of India
DNA was isolated from blood samples obtained from 150 healthy male individuals by standard phenol/chloroform procedure (3). Custom-made primers were labeled with 5'-FAM and JOE fluorescent dyes. PCR amplification for all the eight Y-STRs namely, DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393, was carried out with a 25-ng template DNA in two multiplex reactions, standardized in the laboratory, using primers as described elsewhere (4,5). Amplified PCR products were run on a 5% polyacrylamide gel using a ABI 377 Automated DNA Sequencer. Sizing of the products was done using the ABI 377 Genescan Analysis Software version (3.1).