Volume 48, Issue 1 (January 2003)

    Autosomal Microsatellite Profile of Three Socially Diverse Ethnic Tamil Populations of India

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Blood samples were obtained by venipuncture from random, consenting individuals of the three populations: Kongu Vellala Gounder (56), Irular (54), and Chakkiliyar (49). DNA was isolated using standard phenol/chloroform procedure (1). Fifteen autosomal microsatellite loci were amplified using the PowerPlex® 16 system (Promega Corporation, Madison) according to manufacturer's recommendations (2). The amplified products were separated by denaturing gel electrophoresis using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). Alleles were determined by comparison with the allelic ladder included in the kit.


    Author Information:

    Trivedi, R
    Central Forensic Science Laboratory, West Bengal,

    Sitalaximi, T

    Kashyap, VK
    Central Forensic Science Laboratory, West Bengal,


    Stock #: JFS2002231

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002231

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    Author
    Title Autosomal Microsatellite Profile of Three Socially Diverse Ethnic Tamil Populations of India
    Symposium , 0000-00-00
    Committee E30