ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 1

Allele Frequencies for Two STR Loci D21S1436, D21S2052 in Chinese Population
Ying, BW
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Tang, JP
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Wu, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Yan, J
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Li, YB
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Hou, YP
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Gao, YZ
Institute of Forensic Medicine, Sichuan University (West China University of Medical Sciences,

Abstract

Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d21s1436.htm. The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2,3). Data were analyzed using POWERSTATS program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (5) and no deviation from Hardy-Weinberg equilibrium was observed.

Keywords:
forensic science, Han in Sichuan, China, DNA typing, short tandem repeats, polymerase chain reaction, population genetics, D21S1436, D21S2052

Paper ID: JFS2002219
DOI: 10.1520/JFS2002219
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