Volume 48, Issue 1 (January 2003)
Gene Frequencies for Three Hypervariable DNA Loci in a Chilean Population of Mixed Ancestry
Blood samples were collected in tubes containing ACD from 121 unrelated individuals who asked paternity analysis in The Clinical Hospital of the University of Chile during the years 2000 and 2001. The method presented by Comey (1) was used to remove DNA from the samples. The three STR were amplified using 1 ng of DNA for each PCR reaction, and the fragments electrophoresed through 6% acrylamide gels and silver stained, according to the manufacturer's recommendations (2).