ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 2

Angola Population Data with AmpFℓSTR™ Profiler Plus
Cruz, C
Instituto Nacional de Medicina Legal-Delegação de Lisboa,

Vieira-Silva, C
Instituto Nacional de Medicina Legal-Delegação de Lisboa,

Geada, H

Viriato, L
Instituto Nacional de Medicina Legal-Delegação de Lisboa,

Espinheira, R
Instituto Nacional de Medicina Legal-Delegação de Lisboa,

Ribeiro, T
Instituto Nacional de Medicina Legal-Delegação de Lisboa,

Abstract

Blood stains from 116 unrelated individuals from Angola (West Africa) were stored using bloodstain card and extracted with the standard Chelex method. PCR amplification was performed in a GeneAmp® PCR System 9600, using the AmpFℓSTR® Profiler Plus™ Amplification Kit, according to the manufacturer's recommendations. Electrophoresis of the PCR products was carried out in an ABI Prism™ 377 DNA Sequencer, using 5% denaturing Long Ranger® gels. Sample genotypes were determined with Genotyper® (version 2.0), by comparison with supplied allelic ladders and an internal size standard (GS-500Rox).

Keywords:
forensic science, DNA typing, population genetics, West Africa, short tandem repeats, polymerase chain reaction, Profiler Plus, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820

Paper ID: JFS2002202
DOI: 10.1520/JFS2002202
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