Haplotype Distribution of Nine Y-Chromosome STR-Loci in Two Northern Spanish Populations (Asturias and Aragon)

    Volume 48, Issue 1 (January 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2003

    Page Count: 2


    Budowle, B
    Unit Chief Forensic Science and Training Center, FBI Academy, Quantico, VA

    Martinez-Jarreta, B
    Professor of Forensic Medicine, University of Zaragoza, Zaragoza,

    Abecia, E
    Research scientist, University of Zaragoza, Zaragoza,

    Nievas, P
    Post Doctoral Fellow, University of Zaragoza, Zaragoza,

    Hinojal, R
    Professor of Forensic Medicine, University of Oviedo, Asturias,

    Abstract

    Whole blood samples were collected from healthy unrelated donors born and living in Aragon (n = 136) and Asturias (n = 120). DNA was isolated using standard phenol-cloroform extraction method. Amplification was carried out according to Nievas (1) in a PE 2400 thermocycler (PE Biosystems). Each locus was amplified individually, except DYS389I/II and DYS385 (two loci each with the same set of primers). The recommendations of the International Society for Forensic Genetics were followed for typing and interpretation (2). In addition, we successfully took part in the quality control exercise for Y-STRs organized by Dr. Lutz Roewer, Institute of Legal Medicine, Humboldt University, Berlin, Germany (3). Y-STR haplotype variability was evaluated by means of Analysis of Molecular Variance (AMOVA) (4,5).


    Paper ID: JFS2002201

    DOI: 10.1520/JFS2002201

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    Author
    Title Haplotype Distribution of Nine Y-Chromosome STR-Loci in Two Northern Spanish Populations (Asturias and Aragon)
    Symposium , 0000-00-00
    Committee E30