Developmental Validation of a Single-Tube Amplification of the 13 CODIS STR Loci, D2S1338, D19S433, and Amelogenin: The AmpFℓSTR® Identifiler® PCR Amplification Kit

    Volume 49, Issue 6 (November 2004)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 November 2004

    Page Count: 13


    Leibelt, CS
    Human Identification Group, Foster City, CA

    Reeder, DJ
    Human Identification Group, Foster City, CA

    Hennessy, LK
    Human Identification Group, Foster City, CA

    Foxall, PA
    Human Identification Group, Foster City, CA

    Collins, PJ
    Human Identification Group, Foster City, CA

    Roby, RK
    Human Identification Group, Foster City, CA

    (Received 1 June 2002; accepted 5 June 2004)

    Abstract

    Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, THO1, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFℓSTR® kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFℓSTR® Identifiler® PCR Amplification Kit for human identity and parentage testing.


    Paper ID: JFS2002195

    DOI: 10.1520/JFS2002195

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    Title Developmental Validation of a Single-Tube Amplification of the 13 CODIS STR Loci, D2S1338, D19S433, and Amelogenin: The AmpFℓSTR® Identifiler® PCR Amplification Kit
    Symposium , 0000-00-00
    Committee E30