Volume 49, Issue 6 (November 2004)

    Developmental Validation of a Single-Tube Amplification of the 13 CODIS STR Loci, D2S1338, D19S433, and Amelogenin: The AmpFℓSTR® Identifiler® PCR Amplification Kit

    (Received 1 June 2002; accepted 5 June 2004)

    Published Online: November

    CODEN: JFSOAD

      Format Pages Price  
    PDF 13 $25   ADD TO CART


    Abstract

    Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, THO1, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFℓSTR® kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFℓSTR® Identifiler® PCR Amplification Kit for human identity and parentage testing.


    Author Information:

    Leibelt, CS
    Human Identification Group, Foster City, CA

    Reeder, DJ
    Human Identification Group, Foster City, CA

    Hennessy, LK
    Human Identification Group, Foster City, CA

    Foxall, PA
    Human Identification Group, Foster City, CA

    Collins, PJ
    Human Identification Group, Foster City, CA

    Roby, RK
    Human Identification Group, Foster City, CA


    Stock #: JFS2002195

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002195

    ASTM International
    is a member of CrossRef.

    Author
    Title Developmental Validation of a Single-Tube Amplification of the 13 CODIS STR Loci, D2S1338, D19S433, and Amelogenin: The AmpFℓSTR® Identifiler® PCR Amplification Kit
    Symposium , 0000-00-00
    Committee E30