Journal Published Online: 01 September 2003
Volume 48, Issue 5

AmpFℓSTR® Profiler Plus™ Short Tandem Repeat DNA Analysis of Casework Samples, Mixture Samples, and Nonhuman DNA Samples Amplified under Reduced PCR Volume Conditions (25 µL)

CODEN: JFSCAS

Abstract

As part of the validation of the AmpFℓSTR® Profiler Plus™ short tandem repeat (STR) system, under reduced polymerase chain reaction (PCR) volume conditions (i.e., 25 µL), a total of 275 casework samples were processed. Examples of profiles are presented along with amplification conditions to improve the odds of obtaining balanced and complete profiles for samples showing partial results or profiles with a descending slope. Data collected and used to develop our interpretation guidelines are included. From the mixture studies, full profiles were obtained for minor contributors, using 2 ng of DNA, with ratios of 10:1 or 1:20 and using 1 ng of DNA, with ratios of 10:1 and 1:8. The specificity of the Profiler Plus™ amplification reaction performed in 25 µL was examined and confirmed using a large spectrum of nonhuman DNAs. This report supports the use of the AmpF€STR® Profiler Plus™ STR system for casework DNA typing under reduced PCR volume conditions.

Author Information

Fregeau, CJ
National DNA Data Bank of Canada, Ottawa, Ontario
Bowen, KL
National DNA Data Bank of Canada, Ottawa, Ontario
Leclair, B
National DNA Data Bank of Canada, Ottawa, Ontario Myriad Genetic Laboratories, Salt Lake City, Utah
Trudel, I
National DNA Data Bank of Canada, Ottawa, Ontario
Bishop, L
National DNA Data Bank of Canada, Ottawa, Ontario
Fourney, RM
National DNA Data Bank of Canada, Ottawa, Ontario
Pages: 21
Price: $25.00
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Details
Stock #: JFS2002188
ISSN: 0022-1198
DOI: 10.1520/JFS2002188