Volume 48, Issue 1 (January 2003)

    A Simple Method of DNA Extraction and STR Typing from Urine Samples Using a Commercially Available DNA/RNA Extraction Kit

    (Received 6 September 2002; accepted 24 August 2002)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 µL. Furthermore, application of this DNA extraction procedure to frozen urine samples provided STR allele results comparable to results obtained from fresh samples. Therefore, this extraction procedure is considered to be effective for STR typing of urine samples in both the frozen and aqueous state. Furthermore, addition of sodium azide to fresh urine samples prolonged their storage duration even at room temperature.


    Author Information:

    Takeshita, H
    Gunma University School of Medicine, Gunma,

    Kishi, K
    Gunma University School of Medicine, Gunma,

    Mogi, K
    Gunma University School of Medicine, Gunma,

    Ueki, M
    Fukui Medical University, Fukui,

    Iida, R
    Fukui Medical University, Fukui,

    Nakajima, T
    Gunma University School of Medicine, Gunma,

    Tsukahara, T
    Criminal Investigation Laboratory, Fukui,

    Yasuda, T
    Fukui Medical University, Fukui,

    Kaneko, Y
    Gunma University School of Medicine, Gunma,


    Stock #: JFS2002184

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002184

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    Title A Simple Method of DNA Extraction and STR Typing from Urine Samples Using a Commercially Available DNA/RNA Extraction Kit
    Symposium , 0000-00-00
    Committee E30