ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 3

A Simple Method of DNA Extraction and STR Typing from Urine Samples Using a Commercially Available DNA/RNA Extraction Kit
Takeshita, H
Gunma University School of Medicine,

Kishi, K
Gunma University School of Medicine,

Mogi, K
Gunma University School of Medicine,

Ueki, M
Fukui Medical University,

Iida, R
Fukui Medical University,

Nakajima, T
Gunma University School of Medicine,

Tsukahara, T
Criminal Investigation Laboratory,

Yasuda, T
Fukui Medical University,

Kaneko, Y
Gunma University School of Medicine,

(Received 6 September 2002; accepted 24 August 2002)

Abstract

We devised a simple DNA extraction procedure suitable for STR typing of urine sample. Use of a commercially available DNA/RNA extraction kit equipped with a silica-gel-based membrane made it possible to omit the recovery of urinary nucleated cells by sedimentation before the extraction. Successful genotyping of the TH01, HumTPO and multiplex STRs was achieved using aliquots of urine as small as 100 µL. Furthermore, application of this DNA extraction procedure to frozen urine samples provided STR allele results comparable to results obtained from fresh samples. Therefore, this extraction procedure is considered to be effective for STR typing of urine samples in both the frozen and aqueous state. Furthermore, addition of sodium azide to fresh urine samples prolonged their storage duration even at room temperature.

Keywords:
forensic science, DNA typing, DNA extraction, storage condition, short tandem repeat typing, TH01, HumTPO, multiplex, urine

Paper ID: JFS2002184
DOI: 10.1520/JFS2002184
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