ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 3

DNA Profiling of Azoospermic Semen Samples from Vasectomized Males by Using Y-PLEX™6 Amplification Kit
Schneida, E
ReliaGene Technologies, Inc., LA

Shewale, JG
ReliaGene Technologies, Inc., LA

Sikka, SC
Tulane University, LA

Sinha, SD

(Received 14 August 2002; accepted 28 July 2002)

Abstract

Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX™6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX™6.

Keywords:
forensic science, Y-chromosome, short tandem repeats, DNA typing, human identification, polymerase chain reaction, Y-STR, forensic casework, Y-PLEX, DYS393, DYS19, DYS389II, DYS390, DYS391, DYS385

Paper ID: JFS2002127
DOI: 10.1520/JFS2002127
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