DNA Profiling of Azoospermic Semen Samples from Vasectomized Males by Using Y-PLEX™6 Amplification Kit

    Volume 48, Issue 1 (January 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2003

    Page Count: 3


    Schneida, E
    ReliaGene Technologies, Inc., New Orleans, LA

    Shewale, JG
    ReliaGene Technologies, Inc., New Orleans, LA

    Sikka, SC
    Tulane University, New Orleans, LA

    Sinha, SD

    (Received 14 August 2002; accepted 28 July 2002)

    Abstract

    Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX™6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX™6.


    Paper ID: JFS2002127

    DOI: 10.1520/JFS2002127

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    Title DNA Profiling of Azoospermic Semen Samples from Vasectomized Males by Using Y-PLEX™6 Amplification Kit
    Symposium , 0000-00-00
    Committee E30