Volume 48, Issue 1 (January 2003)

    Development and Validation of a Multiplexed Y-Chromosome STR Genotyping System, Y-PLEX6, for Forensic Casework

    (Received 22 July 2002; accepted 15 July 2002)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    A Y-chromosome multiplex polymerase chain reaction (PCR) amplification kit, known as Y-PLEX™6, has been developed for use in human identification. The Y-PLEX™6 kit enables simultaneous amplification of six polymorphic short tandem repeat (STR) loci located on the non-recombinant region of the human Y-chromosome. These loci are: DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385. Our studies show that as little as 0.2 ng of template DNA can be used for analysis. The specificity of the amplification reaction enabled analysis of male DNA in a male:female DNA mixture at a ratio of 1:125. Among the six Y-STR loci, the maximum mean stutter percentage was 11.9 for allele at DYS389II locus. Attempts at amplification of DNA from various animal sources revealed that the Y-PLEX™6 primers are human specific. Details of the development of the kit, generation and description of the allelic ladders, and validation of the multiplex PCR are presented. In addition, Y-STR allele and haplotype frequencies in three populations have been investigated. The data indicate that results obtained using the Y-PLEX™6 kit are robust, sensitive, and reliable and can be used in human forensic and male lineage identification cases. Erratum to this paper appears in 48(3).


    Author Information:

    Gross, AM
    Minnesota BCA Forensic Science Laboratory, St. Paul, MN

    Budowle, B
    FBI, N.W. Washington, DC

    Schoenbauer, DA
    Minnesota BCA Forensic Science Laboratory, St. Paul, MN

    Shewale, JG
    ReliaGene Technologies, Inc., New Orleans, LA

    Jones, MD
    North Louisiana Criminalistics Laboratory, Shreveport, LA

    Wojtkiewicz, PW
    North Louisiana Criminalistics Laboratory, Shreveport, LA

    Chakraborty, R
    University of Cincinnati, Cincinnati, OH

    Sinha, SK
    ReliaGene Technologies, Inc., New Orleans, LA

    Richey, SL
    ReliaGene Technologies, Inc., New Orleans, LA

    Arcot, SS
    ReliaGene Technologies, Inc., New Orleans, LA


    Stock #: JFS2002068

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002068

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    Title Development and Validation of a Multiplexed Y-Chromosome STR Genotyping System, Y-PLEX6, for Forensic Casework
    Symposium , 0000-00-00
    Committee E30