Volume 48, Issue 1 (January 2003)

    Using Resolution Calculations to Assess Changes in Capillary Electrophoresis Run Parameters

    (Received 12 July 2002; accepted 8 July 2002)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Capillary electrophoresis is widely used in the forensic community for the analysis of Short Tandem Repeat DNA. The CE system used in most forensic laboratories allows the user to modify standard operational protocols to accommodate some samples that fall outside of interpretational guidelines. We have made operational changes and monitored system resolution and the ability of the software to identify alleles as a result of these modifications. Increased amount of amplified product in the injection preparation, lengthened injection times or repeated injections of the same sample were all investigated along with variations in run voltages. Certain protocol modifications offer some advantages to the analysis of STRs and may allow the analyst to avoid extraction of additional portions of crime scene samples to achieve interpretable results.


    Author Information:

    Buel, E
    Vermont Forensic Laboratory, Waterbury, VT

    LaFountain, M
    Vermont Forensic Laboratory, Waterbury, VT

    Schwartz, M
    Vermont Forensic Laboratory, Waterbury, VT


    Stock #: JFS2002018

    ISSN: 0022-1198

    DOI: 10.1520/JFS2002018

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    Author
    Title Using Resolution Calculations to Assess Changes in Capillary Electrophoresis Run Parameters
    Symposium , 0000-00-00
    Committee E30