A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population

    Volume 48, Issue 1 (January 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2003

    Page Count: 6


    Ohtaki, H
    Research student, Nagoya University, Nagoya,

    Mizutani, M
    Research student, Nagoya University, Nagoya,

    Uchihi, R
    Research student, Nagoya University, Nagoya,

    Songsivilai, S
    Professor, Mahidol University, Bangkok,

    Yamamoto, T
    Research student, Nagoya University, Nagoya,

    Yoshimoto, T
    Research student, Nagoya University, Nagoya,

    Waiyawuth, W
    Professor, Mahidol University, Bangkok,

    Katsumata, Y
    Research student, Nagoya University, Nagoya,

    (Received 11 September 2002; accepted 2 September 2002)

    Abstract

    We have previously reported a new triplex amplification and typing system by silver staining for three short tandem repeat (STR) loci, 9q2h2 (D2S3020), D15S233, and D14S299 without "microvariant" alleles such as .1, .2, and, .3 alleles in the Japanese population. In the present study, we established a new quadruplex system with an additional locus D7S809 using primer sets labeled with fluorescent multi-color dyes. Using this system, we genotyped 183 Thai people, found only one “microvariant” allele (allele 20.2) at D7S809, and calculated allele frequencies and some statistical properties at these four STR loci. From these allele frequencies at four STR loci, we performed three statistical analyses including a homozygosity test, a likelihood ratio test, and an exact test for Hardy-Weinberg equilibrium (HWE). Deviations from HWE (p < 0.05) were observed only in the two tests at the locus D7S809.


    Paper ID: JFS2002006

    DOI: 10.1520/JFS2002006

    ASTM International
    is a member of CrossRef.

    Author
    Title A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population
    Symposium , 0000-00-00
    Committee E30