ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 2003
Page Count: 6

A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population
Ohtaki, H
Research student, Nagoya University,

Mizutani, M
Research student, Nagoya University,

Uchihi, R
Research student, Nagoya University,

Songsivilai, S
Professor, Mahidol University,

Yamamoto, T
Research student, Nagoya University,

Yoshimoto, T
Research student, Nagoya University,

Waiyawuth, W
Professor, Mahidol University,

Katsumata, Y
Research student, Nagoya University,

(Received 11 September 2002; accepted 2 September 2002)

Abstract

We have previously reported a new triplex amplification and typing system by silver staining for three short tandem repeat (STR) loci, 9q2h2 (D2S3020), D15S233, and D14S299 without "microvariant" alleles such as .1, .2, and, .3 alleles in the Japanese population. In the present study, we established a new quadruplex system with an additional locus D7S809 using primer sets labeled with fluorescent multi-color dyes. Using this system, we genotyped 183 Thai people, found only one “microvariant” allele (allele 20.2) at D7S809, and calculated allele frequencies and some statistical properties at these four STR loci. From these allele frequencies at four STR loci, we performed three statistical analyses including a homozygosity test, a likelihood ratio test, and an exact test for Hardy-Weinberg equilibrium (HWE). Deviations from HWE (p < 0.05) were observed only in the two tests at the locus D7S809.

Keywords:
forensic science, quadruplex, DNA profiling, STR, population genetics

Paper ID: JFS2002006
DOI: 10.1520/JFS2002006
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