ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 May 2001
Page Count: 3

The Effect of 1,2-Indanedione, a Latent Fingerprint Reagent on Subsequent DNA Profiling
Zamir, A
Forensic Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q.,

Oz, C
Forensic Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q.,

Azoury, M
Latent Fingerprint Development Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q.,

Wiesner, S
Latent Fingerprint Development Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q.,

Abstract

The compound 1,2-indanedione was recently introduced in our laboratory as an operational reagent for developing latent fingerprints on porous surfaces. As part of the reagent implementation, a study was carried out in order to determine whether either of the two operational 1,2-indanediones formulations interferes with further DNA profiling. Both formulations are based on HFE7100 solvent. One is acidic and the other neutral. In a controlled experiment, known donors attached stamps to envelopes by licking them. The stamped envelopes were initially treated with either one indanedione formulation or the other, and DNA was then extracted for STR typing. No differences were observed between the STR profiles obtained from treated and untreated stamps and envelopes, indicating that 1,2-indanedione does not adversely affect the extraction and subsequent amplification of the STRs examined. However, preliminary results indicate that potential DNA analysis depends on the time interval between the indanedione treatment and DNA extraction as no DNA can be recovered six days following treatment. For this reason, it is strongly recommended to extract DNA from treated items of evidence as soon as possible after indanedione treatment.

Keywords:
forensic science, latent fingerprints, 1,2-indanediones, DNA profiling, short tandem repeat (STR), polymerase chain reaction (PCR), THO1, TPOX, CSF1PO, VWA, FESFPS, F13A, D13S317, D7S820, D16S539, saliva, stamp

Paper ID: JFS2001150
DOI: 10.1520/JFS2001150
ASTM International is a member of CrossRef.