Visualization of Postmortem Chondrocyte Damage by Vital Staining and Confocal Laser Scanning 3D Microscopy

    Volume 47, Issue 3 (May 2002)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 May 2002

    Page Count: 4


    Lasczkowski, GE
    Institute of Legal Medicine University of Giessen, Giessen,

    Weiler, G
    Institute of Legal Medicine University of Giessen, Giessen,

    Bratzke, H
    Center of Legal Medicine, University of Frankfurt, Frankfurt/Main,

    Aigner, T
    Institute of Pathology, University of Erlangen-Nürnberg, Erlangen,

    Gamerdinger, U
    Institute of Pathology,University of Giessen, Giessen,

    (Received 11 December 2001; accepted 3 December 2001)

    Abstract

    The present study was designed to investigate whether the combination of vital dyes [calcein acetomethyl ester and ethidium homodimer (LIVE/DEAD(®) Viability/Cytoxicity Kit)] together with confocal laser scanning 3D microscopy was a suitable process to detect postmortem chondrocyte damage, and whether this process could be used to establish postmortem interval.

    Human knee cartilage from 13 autopsies (postmortem interval from 1 day to 2.5 months) was incubated with the two dyes. The chondrocytes revealed intense staining according to their vitality. For those cases that were stored mainly at 4°C there was a vitality of approximately 88 to 96% within the first 4.5 days, which decreased to 58% after 6 days and to 9% after 1.5 months. After 2 days and 14 days at summer temperatures there were 70% and 8% vital chondrocytes respectively. Three of the 13 cases showed that altered body and storage conditions limited the efficacy of the method.

    Initial data suggested a time and temperature dependent increase in cell breakdown. Under stable cooling conditions the use of vital dyes and confocal laser scanning 3D microscopy to measure chondrocyte loss may be a valuable tool for estimating the postmortem interval.


    Paper ID: JFS2000258

    DOI: 10.1520/JFS2000258

    ASTM International
    is a member of CrossRef.

    Author
    Title Visualization of Postmortem Chondrocyte Damage by Vital Staining and Confocal Laser Scanning 3D Microscopy
    Symposium , 0000-00-00
    Committee E30