Use of Nested PCR to Identify Charred Human Remains and Minute Amounts of Blood

    Volume 43, Issue 3 (May 1998)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 May 1998

    Page Count: 5


    Strom, CM
    Illinois Masonic Medical Center, Chicago, IL

    Rechitsky, S
    Illinois Masonic Medical Center, Chicago, IL

    (Received 6 June 1997; accepted 29 September 1997)

    Abstract

    Reliable single cell PCR requires nested or heminested PCR and careful optimization of conditions. This report describes the successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains and a minute quantity of blood that were unsuitable for standard PCR. Use of nested PCR allowed the blood and burned tissue to be identified as human female. Analysis of two PCR length polymorphisms (AMPFLP) was successful on the blood sample and reverse paternity testing yielded a 98% probability that the blood spot was from the victim. The defendant was convicted of murder following a bench trial and the verdict was upheld by the Appellate court.


    Paper ID: JFS16204J

    DOI: 10.1520/JFS16204J

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    Title Use of Nested PCR to Identify Charred Human Remains and Minute Amounts of Blood
    Symposium , 0000-00-00
    Committee E30