ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 May 1998
Page Count: 15

An Illustrated Guide to RFLP Troubleshooting
Riech, AK
Ilinois State Police, Forensic Science Command, IL

Small, AT
Ilinois State Police, Forensic Science Command, IL

Rehnstrom, A
Ilinois State Police, Forensic Science Command, IL

von Beroldingen, C
Oregon State Police Forensic Laboratory, OR

Benzinger, EA
Ohio Bureau of Criminal Identification and Investigation, OH

Emerick, E
Ilinois State Police, Forensic Science Command, IL

Glasner, JA
Ilinois State Police, Forensic Science Command, IL

Boster, KL
Ilinois State Police, Forensic Science Command, IL

Lovekamp, ML
Ilinois State Police, Forensic Science Command, IL

Grigsby, N
Ilinois State Police, Forensic Science Command, IL

Sallee, PJ
Ilinois State Police, Forensic Science Command, IL

Logeman, R
Ilinois State Police, Forensic Science Command, IL

Coons, TM
Oregon State Police Forensic Laboratory, OR

(Received 22 July 1997; accepted 13 October 1997)

Abstract

A large number of reagents and steps are required for restriction fragment length polymorphism (RFLP) analysis, which at times make determining the cause of any observed anomaly difficult. Troubleshooting problems in RFLP analysis is difficult and often the exact cause of a problem cannot be determined. In this paper a collection of controlled experiments detail the consequences of a number of human or materials problems. Although the focus is on forensic applications, this troubleshooting guide will be helpful to anyone employing Southern analysis.

Keywords:
forensic science, DNA typing, restriction fragment length polymorphism, Southern blot, troubleshooting

Paper ID: JFS16200J
DOI: 10.1520/JFS16200J
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