Volume 43, Issue 3 (May 1998)

    Subtyping of the HLA-DQA1 Locus and Independence Testing with PM and STR/VNTR Loci

    (Received 21 April 1997; accepted 27 August 1997)

    Published Online: May

    CODEN: JFSOAD

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    Abstract

    Allele and genotype frequencies for six loci (HLA-DQA1 and PM loci) were determined in African Americans, United States Caucasians, and Southwestern Hispanics. The data include allele frequencies of the HLA-DQA1 4 subtypes. The HLA-DQA1 4 allele subtyping affords greater power of discrimination in African Americans and Southwestern Hispanics than in Caucasians, due to the relatively lower 4.2/4.3 allele frequency in Caucasians. Based on the exact test, all loci, except the GYPA locus in the African American sample (p = 0.011), meet Hardy-Weinberg expectations. There were two examples of significant departures from expectations of independence between alleles of the HLA-DQA1 and PM loci (HBGG/Gc in African Americans, p = 0.30; LDLR/DQA1 in Caucasians, p = 0.023). The HLA-DQA1 and PM loci also were tested for associations with three STR loci and the D1S80 locus. There were four examples of significant departures from expectations of independence (TPOX/D7S8 and THO1/HBGG in African Americans, p = 0.035 and 0.028, respectively; THO1/LDLR in Caucasians, p = 0.028; and GYPA/D1S80 in Hispanics, p = 0.046). The HLA-DQA1 and PM allele frequency data were compared with previously reported data on other sample populations of the same population categories from our laboratory; the allele frequencies at all loci, except the D7S8 locus in Hispanics (p = 0.028), were statistically similar. The frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple locus DNA profile in various general United States populations.


    Author Information:

    Koons, BW
    Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, VA

    Budowle, B
    Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, VA

    Moretti, TR
    Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, VA


    Stock #: JFS16198J

    ISSN: 0022-1198

    DOI: 10.1520/JFS16198J

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    Author
    Title Subtyping of the HLA-DQA1 Locus and Independence Testing with PM and STR/VNTR Loci
    Symposium , 0000-00-00
    Committee E30