ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 May 1998
Page Count: 6

Sex Identification of Elk (Cervus elaphus canadensis), Moose (Alces alces), and White-Tailed Deer (Odocoileus virginianus) Using the Polymerase Chain Reaction
White, BN
Wildlife Forensic DNA Library, Trent University, Ontario

Wilson, PJ

(Received 31 July 1996; accepted 10 September 1997)

Abstract

We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elephus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.

Keywords:
forensic science, polymerase chain reaction, Sry, Fmr-1, elk, moose, white-tailed deer, sex identification, wildlife, DNA typing

Paper ID: JFS16172J
DOI: 10.1520/JFS16172J
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