Volume 43, Issue 3 (May 1998)

    Sex Identification of Elk (Cervus elaphus canadensis), Moose (Alces alces), and White-Tailed Deer (Odocoileus virginianus) Using the Polymerase Chain Reaction

    (Received 31 July 1996; accepted 10 September 1997)

    Published Online: May

    CODEN: JFSOAD

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    Abstract

    We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elephus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.


    Author Information:

    White, BN
    Wildlife Forensic DNA Library, Trent University, Peterborough, Ontario

    Wilson, PJ


    Stock #: JFS16172J

    ISSN: 0022-1198

    DOI: 10.1520/JFS16172J

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    Author
    Title Sex Identification of Elk (Cervus elaphus canadensis), Moose (Alces alces), and White-Tailed Deer (Odocoileus virginianus) Using the Polymerase Chain Reaction
    Symposium , 0000-00-00
    Committee E30