The DRPLA CAG Repeats in an Italian Population Sample: Evaluation of the Polymorphism for Forensic Applications

    Volume 43, Issue 2 (March 1998)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 1998

    Page Count: 3


    Abbondanza, A
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    Maiolini, E
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    Pappalardo, G
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    D'Apote, L
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    Bragliani, M
    DNA analyst and Biologist, Malpighi Hospital Laboratory, Section of molecular and serological typing, Bologna,

    Esposti, PD
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    Pelotti, S
    DNA analyst, Biologist, Ph.D. students and Professor, Section of Legal Medicine, University of Bologna, Bologna,

    Mantovani, V
    DNA analyst and Biologist, Malpighi Hospital Laboratory, Section of molecular and serological typing, Bologna,

    (Received 8 May 1997; accepted 15 August 1997)

    Abstract

    The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found. 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.


    Paper ID: JFS16158J

    DOI: 10.1520/JFS16158J

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    Title The DRPLA CAG Repeats in an Italian Population Sample: Evaluation of the Polymorphism for Forensic Applications
    Symposium , 0000-00-00
    Committee E30