ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 November 2002
Page Count: 1

Allele Frequencies for Two STR Loci D1S1676, D2S2735 in Chinese Population
Ying, BW
Institute of Forensic Medicine, Sichuan University,

Yu, HL
Institute of Forensic Medicine, Sichuan University,

Tang, JP
Institute of Forensic Medicine, Sichuan University,

Wu, J
Institute of Forensic Medicine, Sichuan University,

Yan, J
Institute of Forensic Medicine, Sichuan University,

Zhang, J
Institute of Forensic Medicine, Sichuan University,

Li, YB
Institute of Forensic Medicine, Sichuan University,

Hou, YP
Institute of Forensic Medicine, Sichuan University,

Gao, YZ
Institute of Forensic Medicine, Sichuan University,

Abstract

Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d1s1676.htm. The volume of PCR reaction for each locus was 37.5 μL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data were analyzed using POWERSTATS program (3). The genotype distribution was analyzed for HardyWeinberg equilibrium according to Hou's method (4) and no deviation from Hardy-Weinberg equilibrium was observed.

Keywords:
forensic science, DNA typing, short tandem repeats, polymerase chain reaction, population genetics, D1S1676, D2S2735, Han in Sichuan, China

Paper ID: JFS15586J
DOI: 10.1520/JFS15586J
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