Volume 47, Issue 6 (November 2002)

    Population Study of Seven Microsatellites in Alagoas—Northeastern Brazil

    Published Online: November

    CODEN: JFSOAD

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    Abstract

    Last decade DNA profiling STR loci has become widely used for human identification in forensic science and other related fields. Data banks for STR loci have not yet been established in Alagoas population, Brazil. The aim of this study was to carry out the analysis of allele frequencies of the loci F13B, LPL, D3S1358, D18S51, D21S11, FGA and D10S2325 in Alagoas, as well as to determine other statistics parameters of forensic and paternity interest. Specimens were collected from over 153 unrelated individuals living in Alagoas region—Northeastern Brazil. DNA was obtained from peripheral blood extracted with Chelex method as previously described (1). PCR reactions were performed and analysis of the products was made by polyacrylamide gel electrophoresis silver stained. Allele frequencies and others statistics parameters for forensic and paternity were determined for each locus by the Power Stats software (http://www.promega.com/techserv/apps/hmnid)—Promega Corporation, USA (2). Observed and expected heterozigosity as well as calculations for Hardy-Weinberg Equilibrium were performed in Arlequin 2.0 software (http://lgb.unige.ch/ arlequin) (3). None of the analyzed loci showed deviations from HWE ( p > 0.05) in the population studied. A more complete data set can be accessed at http://www.mhn.ufal.br/forense.


    Author Information:

    Pimentel, BJ
    Research Student, Programa de Identificação Humana e Diagnóstico Molecular, Setor de Biologia, Museu de História Natural, Universidade Federal de Alagoas,

    de Azevedo, DA
    Research Student, Programa de Identificação Humana e Diagnóstico Molecular, Setor de Biologia, Museu de História Natural, Universidade Federal de Alagoas,

    da Silva, ENP
    Research Student, Programa de Identificação Humana e Diagnóstico Molecular, Setor de Biologia, Museu de História Natural, Universidade Federal de Alagoas,

    daSilva, LAF
    Professor, Programa de Identificação Humana e Diagnóstico Molecular, Setor de Biologia, Museu de História Natural, Universidade Federal de Alagoas,

    dos Santos, SS
    Research Student, Programa de Identificação Humana e Diagnóstico Molecular, Setor de Biologia, Museu de História Natural, Universidade Federal de Alagoas,


    Stock #: JFS15583J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15583J

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    Author
    Title Population Study of Seven Microsatellites in Alagoas—Northeastern Brazil
    Symposium , 0000-00-00
    Committee E30