Volume 47, Issue 6 (November 2002)

    Evaluation of Mrna Markers for the Identification of Menstrual Blood

    (Received 11 June 2002; accepted 23 May 2002)

    Published Online: November

    CODEN: JFSOAD

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    Abstract

    The detection of epithelial cells in dried bloodstains by reverse transcriptase-polymerase chain reaction is based on cell- and tissuespecific gene expression. In this paper mRNA markers suitable for the identification of menstrual blood were evaluated. RNA isolated from autopsy tissue samples including endometrium, vaginal mucosa, and blood were screened for tissue-specific expression patterns using RT-PCR with primers for hormone receptors, intermediate filaments, matrix metalloproteinases, heat shock proteins, cytokines, and growth factors. Matrix metalloproteinase (MMP) mRNA could be detected in endometrium but not in blood and other epithelia. This was confirmed in further studies with artificial menstrual bloodstains, indicating that the detection of MMP expression in bloodstains may serve as a forensic marker for menstrual blood.


    Author Information:

    Patzelt, D
    Institute of Legal Medicine, University of Wuerzburg, Wuerzburg,

    Bauer, M
    Institute of Legal Medicine, University of Wuerzburg, Wuerzburg,


    Stock #: JFS15560J

    ISSN: 0022-1198

    DOI: 10.1520/JFS15560J

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    Author
    Title Evaluation of Mrna Markers for the Identification of Menstrual Blood
    Symposium , 0000-00-00
    Committee E30